Molecular diagnostic tests are progressively moving towards lyophilised. There are several advantages for this, including room temperature shipping and storage, extended shelf-life and increased flexibility in sample volume. In order to be compatible with drying however, enzyme preparations must be glycerol-free and include specialised excipients that preserve the mixture as it is exposed to freezing, vacuum and dehydration.
Lyo-Ready qPCR Buffer 2.5x is a glycerol-free qPCR reaction buffer containing lyo-excipients, designed to deliver highly reproducible, accurate qPCR assay results under fast thermal cycling conditions. In order to produce lyophilised, ambient-temperature stable qPCR reagents, dNTP, MgCl2 and glycerol-free Taq DNA polymerase (such as MDX011 or MDX015) must be added prior to lyophilisation.
- Used to create ultra-sensitive qPCR assays
- Formulated with a specialized blend of lyo-excipients, compatible with lyophilisation into beads or cakes
- Minimises non-specific amplification for improved assay sensitivity and reliability
- High efficiency, giving excellent performance in multiplex qPCR assays
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